Composite
Phosphoryl
Part:BBa_K3773514
Designed by: Justin Berg Group: iGEM21_William_and_Mary (2021-10-18)
Circuit to report phosphorylation in vivo
This circuit uses sfGFP to report phosphorylation rates in the cell upon activation of EnvZ in a high osmolarity state. The phosphorylation of EnvZ ultimately causes OmpC production, so we use the ompC promoter in this circuit in order to report phosphorylation of EnvZ.
An increase in green fluorescence is intended to report upregulation of the ompC promoter and therefore increased phosphorylation levels.
BBa_K3773515 is an improved version of this part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 330
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |